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KMID : 0386819950050010038
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Journal of the Institute of Health Environmental Sciences
1995 Volume.5 No. 1 p.38 ~ p.45
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Evaluation of Mutagenic and SOS-inducing Activity of Low Dose X-Ray by Means of the SOS Chromotest
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Abstract
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This study is concerning to comparisons between mutagenicity of low dose X-ray which exists in environment and that of other mutagenic chemicals ymeans of SOS chromotest.
The results are as follows.
SOS chromotest is useful method to screening low dose of X-ray. 45 mGy of X-ray can induce SOS response, so induction factor of SOS chromotest was increased by 23.3% to control. Dose-response curve of X-ray shows positive linear correlation and
no
threshold from.
In all studies dose ranges of X-ray, 4-NQO and MMC, there's no significant variation in alkaline phosphatase activity. this results indicates that such a low concentration can's inhibit overall protein synthesis mechanism in cell. if high dose of
mutagen which can inhibit protein synthesis mechanism were expression, it's need to revise of alkaline phosphatase activity to avoid underestimating of sfiA gene expression. But this studies dose rages of the mutagen didn't affect the total
protein
synthesis, so curve of induction factor activity is similar to that of β-galactosidase activity.
βgalactosidase activity increased according to exposure dose and, as a result, induction factor which was revised by total protein synthesis amount also increased.
Degree of Genotoxicity can be comparable between concentration of mutagenic material by use of SOS chromotest.
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KEYWORD
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